Molecular Basis of Inheritance Practice Test

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What is the role of DNA polymerase in replication?

Catalyzes the formation of the sugar-phosphate backbone by joining nucleotides

DNA polymerase builds the new DNA strand by catalyzing the formation of phosphodiester bonds that join nucleotides into the sugar-phosphate backbone. It reads the template, adds complementary nucleotides to the growing 3' end, and synthesizes in the 5' to 3' direction (requiring a primer to start and often proofreading to correct mistakes). The unwinding of the helix and the creation of RNA primers are handled by helicase and primase, respectively, not by DNA polymerase.

Separates hydrogen bonds between bases

Creates RNA primer

Unwinds DNA

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